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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Evoluce symbiotických bakterií dvoukřídlého krevsajícího hmyzu
 JUHAŇÁKOVÁ, Eliška
This study is focused on bacterial symbionts of blood-sucking insects from the family Hippoboscidae (Diptera), namely Ornithomya biloba and Crataerina pallida. Bacterial symbionts are essential for blood-sucking insects because they provide nutrients absent in blood, particularly vitamin B and cofactors. In this study metagenomic data from Ornithomya biloba and Crataerina pallida were processed by bioinformatic tools and genome drafts were assembled. Genomes of two bacterial symbionts of the genus Arsenophonus were compared with their relatives and levels of genomic modification of these genomes were evaluated. Moreover, the presence of horizontally transferred genes in both Arsenophonus genomes was phylogenetically determined. Finally, metabolic pathways for essential B vitamins and cofactors were reconstructed and compared with other species of the genus Arsenophonus.
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Evolution and genomics of symbionts in Hippoboscidae
 ŠOCHOVÁ, Eva
Obligately blood-sucking parasites harbour symbiotic bacteria providing them B-vitamins and cofactors missing from their blood diet. Within Hippoboscoidea group (parasites of birds and mammals), tsetse flies as medically important vectors have been studied extensively while bat flies and louse flies tend to be neglected. This thesis is composed of two complementary manuscripts focused on phylogeny and origin of bacterial symbionts in Hippoboscidae family (manuscript 1) and their genome evolution (manuscript 2). First, phylogenetic approach was employed to determine lineages of obligate and facultative symbionts present in this group. Second, genomic and phylogenomic analyses were carried out to better understand evolution of obligate endosymbionts from the Arsenophonus genus in this group. Results of the two studies indicate that relationships between Hippoboscoidea and their symbionts are extremely dynamic with frequent replacements of obligate symbionts. This hypothesis is supported by both phylogenetic and genomic evidence, in particular, Arsenophonus endosymbionts of Hippoboscidae represent several distinct lineages (of likely different ages) with noticeable differences in genome features and metabolic capabilities. The data presented in this thesis thus greatly extend our knowledge about evolution and genomics of symbiotic bacteria in Hippoboscidae and bloodsucking hosts in general.
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